Study 10
Name of Study: Activite Angioprotectrice Des Oligomeres Procyanidoliques Chez L'animal/Oedeme De La Patte (Angioprotective Activity Of Oligomeric Proanthocyanidins In Animals/Paw Oedema)
Conducted by: Barbier, Maffraud, Savi, Unkovic Vilain
Where study conducted: Sanofi-Recherche, Montpellier, France
Material used for study: Masquelier's OPC extract from Vitis Vinifera (grape seeds)
In-vivo study: Yes
Category of animal subjects: 14 male Sprague Dawley rats
Dosages used: 25, 50, 100, 200mg/kg i.p.
Summary/Abstract: The results obtained with regard to carragenin induced oedema after administration of OPC by oral way are represented in Table I. These results show that the OPC at 500mg/kg cause a neat reduction of the carragenin provoked oedema. The thus obtained reduction is significant for times 2 and 3 hours after injection of the phlogogen.
By intraperitoneal way the effect of OPC is even more marked. It manifests itself just as good in cases of Carragenin, Serotonin, Histamine and Formol induced oedema. Table II presents efficient intraperitoneal dosages to reach 50% inhibition (E.D. 50) with regard to each of the phlogogens.
Carragenin: 55mg/kg i.p.
Serotonin: 45mg/kg i.p.
Histamin: 40mg/kg i.p.
Formol: 150mg/kg i.p.
(Carragenin: 0.1 ml of a solution of Carragein (Cummings) et 1% in physiological solution. This test was also performed using as phlogogen agents Serotonin (2mg), Histamine (20mg) and Formol (20mg)