Study 1
Name of Study: Inhibition Of Angiotensin I Converting Enzyme By Flavanolic Compounds: In-Vitro And In-Vivo Studies
Conducted by: Marie-Therese Meunier, Francoise Villie, Madelaine Jonadet, Janine Bestide and Pierre Bastide
Published in: Planta Medica
Publication date: 1987
Where study conducted: Laboratoire de Chimie Biologique et de Pharmacodynamie and CNRS, Faculte de Pharmacie, Clermont-Ferrand, France
Material used for study: Masquelier's OPC extract from Vitis Vinifera (grape seeds)
In-vitro study: Yes
Summary/Abstract: The procyanidolic oligomers coming from Vitis Vinifera L. (two fractions) and Cupressus sompervirens L. (three fractions), and the monomers, (-) catechins, (-) epicatechin were tested for their effects on angiotensin I converting enzyme (ACE) activity. The oligomers are the most active (dosc I50 of 0.08mg/ml for the fraction A of Vitis, the most active substance). Monomers have little activity. In-vivo, the vasopressive response to Ang 1 is inhibited by approximately 20% to 40% in 5mg/kg i.v.). a Il also is inhibited which suggests another action, perhaps due to the forming of compounds between Ang 1 and Ang 11 and the oligomers.
Acknowledgments: The Authors would like to thank M. Masquelier (Dean) for providing the procyanidolic oligomers of Vitis Vinifera L. (Endotelon) and the Dulcis Laboratories for the fractions of Cupressus sempervirens L. (Vascuton). This work was carried out with the technical collaboration of Elaine Duroux.